CIM® r-Protein L-0.34 mL is a shallow, disk-shaped monolith with a column volume of 0.34 mL. It is predominantly suitable for initial screening and method development. The CIM® r-Protein L-0.34 mL disk needs to be inserted into a housing and is immediately ready for use. The r-Protein L (recombinant protein L produced in E. coli) is an affinity ligand, used for fast, highly efficient purification of different immunoglobulins. In contrast to the protein A and protein G, the r-protein L binds to the kappa light chain of antibodies and as such it can be specifically used for IgG, IgA, and IgM antibodies containing only kappa chains. Species whose antibodies contain almost exclusively lambda chains (bovine, goat, sheep, and horse) will not bind well to the Protein L.
Read more: axial flow, Conjoint Liquid Chromatography (CLC)