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Fast purification/concentration of a bacteriophage T4 by using short monolithic columns

Bacteriophage T4 was harvested from E.coli suspension. The clarified and 0.22µm filtered solution was subjected to single step separation on CIM® QA Disk. An optimized stepwise elution gradient resulted in 78% recovery of infective bacteriophage in the elution fraction, while efficient separation of phages from DNA and proteins was obtained.

CIMmultus™ QA-1 Advanced Composite Column (Quaternary amine) (Pores 2 um)Information
CIMmultus™ DEAE-1 Advanced Composite Column (Diethylamino) (Pores 2 um)Information

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