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pDNA purification training

pDNAThe plasmid DNA purification training with CIMmultus™ columns includes lectures on pDNA purification and practical hands-on work: from bacterial lysate to pure pDNA, standard quantification methods, and CIMac™ pDNA column-based analytics.

Practical hands-on work will cover the following topics:

  • Sample preparation for efficient purification of pDNA on a CIMmultus™ DEAE column:
    – Screening of different alkaline lysis conditions
    – Determination of optimal conditions for RNA precipitation
    – Screening experiments to determine the optimal conductivity of the loading material for efficient pDNA purification on a CIMmultus™ DEAE column
  • Purification of pDNA on a weak anion exchanger (CIMmultus™ DEAE column)
  • Screening experiment to determine optimal conditions for the separation of oc and sc pDNA on a CIMmultus™ C4 HLD column
  • Separation of oc and sc pDNA isoform on CIMmultus™ C4 HLD column
  • Standard quantification methods (agarose gel electrophoresis, DNA and protein quantification)and CIMac™ pDNA column-based analytics will be used throughout the training.

Please contact us to arrange dates of your training at This email address is being protected from spambots. You need JavaScript enabled to view it. or through your local BIA Separations' sales representative.