Next generation biotechnology products demand next generation production materials. Monolithic chromatographic supports mark the beginning of a new generation of downstream processing. Monoliths are enabling tools for purification of biomolecules in nanometer range (virus, plasmid DNA, phages, IgM, PEGylated proteins, etc.) that offer unprecedented possibilities. They stand for elaborate design of high speed, high efficiency, and high yield in downstream processing.
...monolithic columns will, some day, become the main workhorse in chromatographic separation.
Prof. George Guiochon, J.Chrom. 2007
Monoliths (monos [Greek]: one, single and lithos [Greek]: stone) are stationary phases cast as a single piece, containing interconnected flow channels with defined diameter. Mass transfer of target molecules within these channels is exclusively governed by convective flow of the mobile phase.
The absence of pores, which are dominated by restricting pore diffusion, makes monoliths superior in terms of:
Due to convective flow in all channels resolution is independent of flow rate. Resolution remains consistent over a wide range of operational speed – as high as your molecule tolerates.
Convective flow in all channels eliminates mass transfer restrictions due to slow pore diffusion. The speed of the mobile phase governs the speed of the separation time.
Appropriate channel diameter and direct access to binding sites provide high binding capacity – especially for large biomolecules such as viruses, virus like particles, DNA, IgX, PEGylated proteins, etc.
Monoliths are purpose designed chromatographic materials for fast, efficient but gentle purification of your next generation bio drug.