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Reproducibility and Lifetime of Novel Ion-Exchange Monolithic Columns Intended for PAT and QA/QC of Biomacromolecules

Monoliths represent a novel generation of chromatographic stationary phases. Despite many advantages of the monoliths over the conventional stationary phases for the separation of biomacromolecules, such as low back-pressure, flow unaffected chromatographic properties and high resolution power they are not often found in commercial chromatographic products. One of the general beliefs is that it is challenging to prepare different monolith column batches of the same chemistry with have identical physico-chemical characteristics.


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