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Application of Convective Interaction Media Disks with Immobilised Glucose Oxidase for On-line Glucose Measurements

M. Vodopivec, M. Berovič, J. Jančar, A. Podgornik, A. Štrancar

Analytica Chimica Acta 407 (2000) 105-110

A new polymeric macroporous material, Convective Interaction Media (CIM) was applied as a support for glucose oxidase (GOD) immobilisation. CIM epoxy disks with the immobilised enzyme were integrated as an enzyme reactor in a flow injection analysis (FIA) system and applied to on-line monitoring of glucose during cultivation of Saccharomyces cerevisiae and citric acid production by Aspergillus niger. The developed CIM GOD disk–FIA system exhibited good signal reproducibility and satisfactory long-term stability with a linear response in the range 10–200 mg l-1. The CIM disk applied as an enzyme reactor proved to be a successful replacement for conventionally used packed-bed columns and as such it was well suited for on-line monitoring of bioprocesses.

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