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Dissociation and fractionation of heavy and light chains from IgG monoclonal antibody

P. Gagnon, G. Rodriquez, S. Zaidi

Journal of Chromatography A, 1218 (2011) 2402-2404

A basic method for dissociation and fractionation of monoclonal IgG heavy and light chain is described. It employs less noxious and hazardous reagents than the classical mercaptoethanol/propionic acid process and replaces size exclusion chromatography with cation exchange on a monolith to improve productivity. Significant scope remains to refine the conditions. The method can be applied to other disulfide bonded proteins with significant affinity for cation exchangers.

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