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High-Performance Membrane Chromatography: A Novel Method of Protein Separation

T. B. Tennikova, B. G. Belenkii, F. Švec

Journal of Liquid Chromatography, 13(1), 63-70 (1990)

Basing on the fact that only short layers of a chromatographic column contribute to the separation in the interaction chromatography, 1 mm thick membranes from macroporous methacrylate polymer provided with functional groups were synthetized and used for protein separation. The chromatograms show that the separation is fully comparable with that experienced on a filled column but the advantage of a membrane is up to two orders of magnitude lower pressure during the process and very high loading reaching up to 40 g/m2. This recommends the high performance membrane chromatography also for large scale preparative separations.

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